DNA

Part:BBa_K2985160:Design

Designed by: Diqing Sheng   Group: iGEM19_Jiangnan-China   (2019-10-15)


P43-liaH->Lipopeptide tolerance gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 149
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 149
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 149
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 149
    Illegal NgoMIV site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If the bacterial cell had the gene sequence, it would be much easier to use the composite part.


Source

Bacillus subtilis subsp. subtilis str. 168

References